vari fluor 4 color multiplex if kit (MedChemExpress)
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vari fluor 4 color multiplex if kit
Vari Fluor 4 Color Multiplex If Kit, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiple+kit/Vari+Fluor+4-colour+Multiple+Fluorescent+Staining+Kit/pm42882463-100-27-34
Average 94 stars, based on 1 article reviews
Vari Fluor 4 Color Multiplex If Kit, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiple+kit/Vari+Fluor+4-colour+Multiple+Fluorescent+Staining+Kit/pm42882463-100-27-34
Average 94 stars, based on 1 article reviews
vari fluor 4 color multiplex if kit - by Bioz Stars,
2026-11
94/100 stars
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Staining:Article Title: Qigui didang decoction alleviates renal injury in a diabetic kidney disease model with metabolic memory features: association with ferroptosis and the SIRT1/Nrf2 pathway Article Snippet: .. Reagents and Kits: Metformin (Solarbio, Cat# D9351); STZ (Solarbio, Cat# S8050); Hematoxylin (Servicebio, Cat# G1004); Eosin (Solarbio, Cat# G1100); Masson’s trichrome staining kit (Servicebio, Cat# G1006); Prussian blue staining kit (Solarbio, Cat# G1422); Modified Oil Red O staining kit (Solarbio, Cat# G1263); Assay kits for reduced glutathione (GSH, Nanjing Jiancheng Bioengineering Institute, Cat# A006-1-1), total cholesterol (TCH/T-CHO, Cat# A111-1-1), triglycerides (TG, Cat# A110-1-1), total superoxide dismutase (T-SOD, Cat# A001-3-2), malondialdehyde (MDA, Cat# A003-1-2), blood urea nitrogen (BUN, Cat# C013-2-1), creatinine (Cr, Cat# C011-2-1), high-density lipoprotein cholesterol (HDL-C, Cat# A112-1-1), low-density lipoprotein cholesterol (LDL-C, Cat# A113-1-1), urinary protein quantification (Cat# F006-1-1), and cystatin C (Cys-C, Cat# C035-2-1); Ferrous ion detection kit (Solarbio, Cat# BC5415); Article Title: BAP31 Facilitates TGF-β Trafficking and Secretion and Promotes Dendritic Cell Dysfunction in Cervical Cancer. Article Snippet: .. Cells were washed three times with PBS before fixation with 4% paraformaldehyde and then permeabilized with 0.2% Triton X-100 for 10 min. Staining was performed using the Article Title: An antibody-PROTAC conjugate targets BRD4/c-Myc/PD-L1 to enhance immunotherapy efficacy in triple-negative breast cancer. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antifade mounting medium (with DAPI) Beyotime Cat#P0131 DMSO Beyotime Cat#ST038 Lipofectamine 3000 transfection reagent Thermo Fisher Scientific Cat#L3000015 Fixable Viability Dye eFluorTM 780 Thermo Fisher Scientific Cat#65-0865-14 Puromycin Beyotime Cat#ST551 DNase I Beyotime Cat#D7076 Collagenase Type IV Sigma Cat#C4-28 Percoll Sigma Cat#P4937 Cell Stimulation Cocktail (plus protein transport inhibitors) (500×) Thermo Fisher Scientific Cat#00-4975-93 Foxp3/Transcription Factor Staining Fixation/ Permeabilization Buffer Thermo Fisher Scientific Cat#00-5523-00 Modification:Article Title: Qigui didang decoction alleviates renal injury in a diabetic kidney disease model with metabolic memory features: association with ferroptosis and the SIRT1/Nrf2 pathway Article Snippet: .. Reagents and Kits: Metformin (Solarbio, Cat# D9351); STZ (Solarbio, Cat# S8050); Hematoxylin (Servicebio, Cat# G1004); Eosin (Solarbio, Cat# G1100); Masson’s trichrome staining kit (Servicebio, Cat# G1006); Prussian blue staining kit (Solarbio, Cat# G1422); Modified Oil Red O staining kit (Solarbio, Cat# G1263); Assay kits for reduced glutathione (GSH, Nanjing Jiancheng Bioengineering Institute, Cat# A006-1-1), total cholesterol (TCH/T-CHO, Cat# A111-1-1), triglycerides (TG, Cat# A110-1-1), total superoxide dismutase (T-SOD, Cat# A001-3-2), malondialdehyde (MDA, Cat# A003-1-2), blood urea nitrogen (BUN, Cat# C013-2-1), creatinine (Cr, Cat# C011-2-1), high-density lipoprotein cholesterol (HDL-C, Cat# A112-1-1), low-density lipoprotein cholesterol (LDL-C, Cat# A113-1-1), urinary protein quantification (Cat# F006-1-1), and cystatin C (Cys-C, Cat# C035-2-1); Ferrous ion detection kit (Solarbio, Cat# BC5415); Article Title: QD394 induces ferroptosis and suppresses the proliferation of colorectal cancer via the SP1/JNK pathway. Article Snippet: 1 Department of Gastroenterology, Yanbian University Hospital, Yanji 133002, China 2 Central Laboratory, Yanbian University Hospital, Yanji 133002, China Abstract Lipid peroxidation triggers ferroptosis, a type of regulated cell death that is iron-dependent.. Owing to its important role in tumor suppression, ferroptosis represents an extremely promising therapeutic target for cancer.. QD394, a quinazolinonebased compound, was recently identified as a novel redox regulator with demonstrated cytotoxic and proapoptotic effects in pancreatic and breast cancer models. Multiple Displacement Amplification:Article Title: Qigui didang decoction alleviates renal injury in a diabetic kidney disease model with metabolic memory features: association with ferroptosis and the SIRT1/Nrf2 pathway Article Snippet: .. Reagents and Kits: Metformin (Solarbio, Cat# D9351); STZ (Solarbio, Cat# S8050); Hematoxylin (Servicebio, Cat# G1004); Eosin (Solarbio, Cat# G1100); Masson’s trichrome staining kit (Servicebio, Cat# G1006); Prussian blue staining kit (Solarbio, Cat# G1422); Modified Oil Red O staining kit (Solarbio, Cat# G1263); Assay kits for reduced glutathione (GSH, Nanjing Jiancheng Bioengineering Institute, Cat# A006-1-1), total cholesterol (TCH/T-CHO, Cat# A111-1-1), triglycerides (TG, Cat# A110-1-1), total superoxide dismutase (T-SOD, Cat# A001-3-2), malondialdehyde (MDA, Cat# A003-1-2), blood urea nitrogen (BUN, Cat# C013-2-1), creatinine (Cr, Cat# C011-2-1), high-density lipoprotein cholesterol (HDL-C, Cat# A112-1-1), low-density lipoprotein cholesterol (LDL-C, Cat# A113-1-1), urinary protein quantification (Cat# F006-1-1), and cystatin C (Cys-C, Cat# C035-2-1); Ferrous ion detection kit (Solarbio, Cat# BC5415); Article Title: QD394 induces ferroptosis and suppresses the proliferation of colorectal cancer via the SP1/JNK pathway. Article Snippet: 1 Department of Gastroenterology, Yanbian University Hospital, Yanji 133002, China 2 Central Laboratory, Yanbian University Hospital, Yanji 133002, China Abstract Lipid peroxidation triggers ferroptosis, a type of regulated cell death that is iron-dependent.. Owing to its important role in tumor suppression, ferroptosis represents an extremely promising therapeutic target for cancer.. QD394, a quinazolinonebased compound, was recently identified as a novel redox regulator with demonstrated cytotoxic and proapoptotic effects in pancreatic and breast cancer models. Article Title: A bispecific nanobody-drug conjugate targeting TROP2 and c-Met for low-concentration, single-dose treatment of pancreatic cancer. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alexa Fluor® 700-anti human TROP2 antibody R&D Systems Cat#FAB650N-100UG; RRID: AB_3651666 PE-anti human c-Met antibody Abcam Cat#ab279587; Temporary RRID:AB_3740207 TACSTD2/TROP2 Monoclonal antibody Wuhan Sanying Cat#68141-1-Ig; CloneNo.5A6B1; RRID: AB_3085043 c-Met (Cytoplasmic) Polyclonal antibody Wuhan Sanying Cat#25869-1-AP; RRID: AB_2880276 Goat anti-human IgG Fc (HRP) Abcam Cat#ab97225; RRID: AB_10680850 HRP conjugated Goat Anti-Mouse IgG (H + L) Servicebio Cat#GB23301; RRID: AB_2904020 HRP conjugated Goat Anti-Rabbit IgG (H + L) Servicebio Cat#GB23303; RRID: AB_2811189 DyLight 650-Goat anti human IgG Fc Thermo Fisher RRID: AB_2556717 Rabbit anti-MMAE antibody(YA899) MedChemExpress (MCE) RRID: AB_3103046 F(ab’)2-Goat anti-Rabbit IgG (H + L) CrossAdsorbed Secondary Antibody, Alexa Fluor TM 647 Thermo Fisher RRID: AB_2535814 Goat anti-Human IgG (H + L) CrossAdsorbed Secondary Antibody, Alexa Fluor TM 488 Thermo Fisher RRID: AB_2534080 Cleaved Caspase-3 (Asp175) Antibody #9661Cleaved-Caspase3 Cell Signaling Technology (CST) Cat#9661S; RRID: AB_2341188 Cleaved Caspase-9 (Asp330) (D2D4) Rabbit mAb #7237 Cell Signaling Technology (CST) Cat#7237T; RRID: AB_10895832 Recombinant Anti-Caspase-7 antibody (Mouse mAb) Servicebio Cat#GB15540-100; Temporary RRID: AB_3740208 Anti-Cleaved-Caspase-3 Rabbit pAb Servicebio Cat#GB11532-100; RRID: AB_3096316 Anti-Cleaved PARP1 Rabbit pAb Servicebio Cat#GB111503-100; RRID: AB_3711714 Anti-Ki67 Rabbit pAb Servicebio Cat#GB111499-100; RRID: AB_2927572 Anti-PCNA Mouse mAb Servicebio Cat#GB12010-100; RRID: AB_3714765 Bacterial and virus strains DH5α Competent Cells Shenzhen Comedi Life Sciences Cat#KTSM101L ER2738 Electrocompetent Cells Lucigen Cat#60522-1 Biological samples Multi-Organ Cancer Tissue Microarray (TMA) Shanghai Outdo Biotech Cat#HOrgC180PG01-1 Pancreatic Cancer Tissue Microarray (TMA) Shanghai Outdo Biotech Cat#HPanA120PG02 Normal Human Multi-Organ Tissue Microarray (TMA) Shanghai Outdo Biotech Cat#HOrgN120PT02 Patient-derived xenografts (PDX) Nanchang Royo Biotech Patient ID: RYP-748; Patient ID: RYP-1301 Chemicals, peptides, and recombinant proteins Monomethyl auristatin E(MMAE) MedChemExpress (MCE) HY-15162; CAS No.474645-27-7 mc-vc-PABC-MMAE MedChemExpress (MCE) HY-15575; CAS No.646502-53-6 Cyanine5.5 NHS ester Luminprobe Cat#47020 DyLight TM 633 NHS Ester Thermo Fisher Cat#46414 Beetle Luciferin, Potassium Salt Promega Cat#E1601 (Continued on next page) Cell Reports Medicine 7, 102688, April 21, 2026 e1 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Phalloidin Labeling Probes Thermo Fisher Scientific Cat#A12379 PageRuler TM Prestained Protein Ladder Thermo Fisher Scientific Cat#26616 Sacituzumab govitecan MedChemExpress (MCE) HY-132254; CAS No.1491917-83-9 Telisotuzumab vedotin MedChemExpress (MCE) HY-141601; CAS No.1714088-51-3 Cathepsin B Protein, Human (His) MedChemExpress (MCE) Cat#HY-P70830 TROP2 Protein, Human, Recombinant (His Tag) Sinobiological Cat#10428-H08H c-MET Protein, Human, Recombinant (ECD, His Tag) Sinobiological Cat#10692-H08H Critical commercial assays Opal 7-color Manual IHC Kit BIOMART.CN Cat#NEL811001KT Article Title: An antibody-PROTAC conjugate targets BRD4/c-Myc/PD-L1 to enhance immunotherapy efficacy in triple-negative breast cancer. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antifade mounting medium (with DAPI) Beyotime Cat#P0131 DMSO Beyotime Cat#ST038 Lipofectamine 3000 transfection reagent Thermo Fisher Scientific Cat#L3000015 Fixable Viability Dye eFluorTM 780 Thermo Fisher Scientific Cat#65-0865-14 Puromycin Beyotime Cat#ST551 DNase I Beyotime Cat#D7076 Collagenase Type IV Sigma Cat#C4-28 Percoll Sigma Cat#P4937 Cell Stimulation Cocktail (plus protein transport inhibitors) (500×) Thermo Fisher Scientific Cat#00-4975-93 Foxp3/Transcription Factor Staining Fixation/ Permeabilization Buffer Thermo Fisher Scientific Cat#00-5523-00 Enzyme-linked Immunosorbent Assay:Article Title: Qigui didang decoction alleviates renal injury in a diabetic kidney disease model with metabolic memory features: association with ferroptosis and the SIRT1/Nrf2 pathway Article Snippet: .. Reagents and Kits: Metformin (Solarbio, Cat# D9351); STZ (Solarbio, Cat# S8050); Hematoxylin (Servicebio, Cat# G1004); Eosin (Solarbio, Cat# G1100); Masson’s trichrome staining kit (Servicebio, Cat# G1006); Prussian blue staining kit (Solarbio, Cat# G1422); Modified Oil Red O staining kit (Solarbio, Cat# G1263); Assay kits for reduced glutathione (GSH, Nanjing Jiancheng Bioengineering Institute, Cat# A006-1-1), total cholesterol (TCH/T-CHO, Cat# A111-1-1), triglycerides (TG, Cat# A110-1-1), total superoxide dismutase (T-SOD, Cat# A001-3-2), malondialdehyde (MDA, Cat# A003-1-2), blood urea nitrogen (BUN, Cat# C013-2-1), creatinine (Cr, Cat# C011-2-1), high-density lipoprotein cholesterol (HDL-C, Cat# A112-1-1), low-density lipoprotein cholesterol (LDL-C, Cat# A113-1-1), urinary protein quantification (Cat# F006-1-1), and cystatin C (Cys-C, Cat# C035-2-1); Ferrous ion detection kit (Solarbio, Cat# BC5415); TUNEL Assay:Article Title: Qigui didang decoction alleviates renal injury in a diabetic kidney disease model with metabolic memory features: association with ferroptosis and the SIRT1/Nrf2 pathway Article Snippet: .. Reagents and Kits: Metformin (Solarbio, Cat# D9351); STZ (Solarbio, Cat# S8050); Hematoxylin (Servicebio, Cat# G1004); Eosin (Solarbio, Cat# G1100); Masson’s trichrome staining kit (Servicebio, Cat# G1006); Prussian blue staining kit (Solarbio, Cat# G1422); Modified Oil Red O staining kit (Solarbio, Cat# G1263); Assay kits for reduced glutathione (GSH, Nanjing Jiancheng Bioengineering Institute, Cat# A006-1-1), total cholesterol (TCH/T-CHO, Cat# A111-1-1), triglycerides (TG, Cat# A110-1-1), total superoxide dismutase (T-SOD, Cat# A001-3-2), malondialdehyde (MDA, Cat# A003-1-2), blood urea nitrogen (BUN, Cat# C013-2-1), creatinine (Cr, Cat# C011-2-1), high-density lipoprotein cholesterol (HDL-C, Cat# A112-1-1), low-density lipoprotein cholesterol (LDL-C, Cat# A113-1-1), urinary protein quantification (Cat# F006-1-1), and cystatin C (Cys-C, Cat# C035-2-1); Ferrous ion detection kit (Solarbio, Cat# BC5415); Bicinchoninic Acid Protein Assay:Article Title: Qigui didang decoction alleviates renal injury in a diabetic kidney disease model with metabolic memory features: association with ferroptosis and the SIRT1/Nrf2 pathway Article Snippet: .. Reagents and Kits: Metformin (Solarbio, Cat# D9351); STZ (Solarbio, Cat# S8050); Hematoxylin (Servicebio, Cat# G1004); Eosin (Solarbio, Cat# G1100); Masson’s trichrome staining kit (Servicebio, Cat# G1006); Prussian blue staining kit (Solarbio, Cat# G1422); Modified Oil Red O staining kit (Solarbio, Cat# G1263); Assay kits for reduced glutathione (GSH, Nanjing Jiancheng Bioengineering Institute, Cat# A006-1-1), total cholesterol (TCH/T-CHO, Cat# A111-1-1), triglycerides (TG, Cat# A110-1-1), total superoxide dismutase (T-SOD, Cat# A001-3-2), malondialdehyde (MDA, Cat# A003-1-2), blood urea nitrogen (BUN, Cat# C013-2-1), creatinine (Cr, Cat# C011-2-1), high-density lipoprotein cholesterol (HDL-C, Cat# A112-1-1), low-density lipoprotein cholesterol (LDL-C, Cat# A113-1-1), urinary protein quantification (Cat# F006-1-1), and cystatin C (Cys-C, Cat# C035-2-1); Ferrous ion detection kit (Solarbio, Cat# BC5415); Article Title: An antibody-PROTAC conjugate targets BRD4/c-Myc/PD-L1 to enhance immunotherapy efficacy in triple-negative breast cancer. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antifade mounting medium (with DAPI) Beyotime Cat#P0131 DMSO Beyotime Cat#ST038 Lipofectamine 3000 transfection reagent Thermo Fisher Scientific Cat#L3000015 Fixable Viability Dye eFluorTM 780 Thermo Fisher Scientific Cat#65-0865-14 Puromycin Beyotime Cat#ST551 DNase I Beyotime Cat#D7076 Collagenase Type IV Sigma Cat#C4-28 Percoll Sigma Cat#P4937 Cell Stimulation Cocktail (plus protein transport inhibitors) (500×) Thermo Fisher Scientific Cat#00-4975-93 Foxp3/Transcription Factor Staining Fixation/ Permeabilization Buffer Thermo Fisher Scientific Cat#00-5523-00 Formalin-fixed Paraffin-Embedded:Article Title: Potentiating BSEP-mediated bile acid efflux reverses first-line tyrosine kinase inhibitor resistance in hepatocellular carcinoma. Article Snippet: Tyrosine kinase inhibitor (TKI) resistance limits therapy for hepatocellular carcinoma (HCC).. Integrating RNAseq and public cohort data, we found consistent downregulation of the bile salt export pump (BSEP/ABCB11) in TKI-resistant HCC associated with poorer prognosis and reduced clinical response.. Functional in vitro and xenograft studies, using BSEP overexpression/knockdown and TKI-resistant cell lines plus targeted metabolomics, showed BSEP expression deficiency leads to intracellular accumulation of primary conjugated bile acids (BAs)—especially glycocholic acid (GCA)—which activates EGFR signaling and drives resistance; restoring BSEP enhances BA efflux and resensitizes cells and tumors to TKIs. Tissue:Article Title: Potentiating BSEP-mediated bile acid efflux reverses first-line tyrosine kinase inhibitor resistance in hepatocellular carcinoma. Article Snippet: Tyrosine kinase inhibitor (TKI) resistance limits therapy for hepatocellular carcinoma (HCC).. Integrating RNAseq and public cohort data, we found consistent downregulation of the bile salt export pump (BSEP/ABCB11) in TKI-resistant HCC associated with poorer prognosis and reduced clinical response.. Functional in vitro and xenograft studies, using BSEP overexpression/knockdown and TKI-resistant cell lines plus targeted metabolomics, showed BSEP expression deficiency leads to intracellular accumulation of primary conjugated bile acids (BAs)—especially glycocholic acid (GCA)—which activates EGFR signaling and drives resistance; restoring BSEP enhances BA efflux and resensitizes cells and tumors to TKIs. Multiplex Assay:Article Title: Potentiating BSEP-mediated bile acid efflux reverses first-line tyrosine kinase inhibitor resistance in hepatocellular carcinoma. Article Snippet: Tyrosine kinase inhibitor (TKI) resistance limits therapy for hepatocellular carcinoma (HCC).. Integrating RNAseq and public cohort data, we found consistent downregulation of the bile salt export pump (BSEP/ABCB11) in TKI-resistant HCC associated with poorer prognosis and reduced clinical response.. Functional in vitro and xenograft studies, using BSEP overexpression/knockdown and TKI-resistant cell lines plus targeted metabolomics, showed BSEP expression deficiency leads to intracellular accumulation of primary conjugated bile acids (BAs)—especially glycocholic acid (GCA)—which activates EGFR signaling and drives resistance; restoring BSEP enhances BA efflux and resensitizes cells and tumors to TKIs. Article Title: BAP31 Facilitates TGF-β Trafficking and Secretion and Promotes Dendritic Cell Dysfunction in Cervical Cancer. Article Snippet: .. Cells were washed three times with PBS before fixation with 4% paraformaldehyde and then permeabilized with 0.2% Triton X-100 for 10 min. Staining was performed using the Fluorescence:Article Title: Potentiating BSEP-mediated bile acid efflux reverses first-line tyrosine kinase inhibitor resistance in hepatocellular carcinoma. Article Snippet: Tyrosine kinase inhibitor (TKI) resistance limits therapy for hepatocellular carcinoma (HCC).. Integrating RNAseq and public cohort data, we found consistent downregulation of the bile salt export pump (BSEP/ABCB11) in TKI-resistant HCC associated with poorer prognosis and reduced clinical response.. Functional in vitro and xenograft studies, using BSEP overexpression/knockdown and TKI-resistant cell lines plus targeted metabolomics, showed BSEP expression deficiency leads to intracellular accumulation of primary conjugated bile acids (BAs)—especially glycocholic acid (GCA)—which activates EGFR signaling and drives resistance; restoring BSEP enhances BA efflux and resensitizes cells and tumors to TKIs. Immunohistochemistry:Article Title: Potentiating BSEP-mediated bile acid efflux reverses first-line tyrosine kinase inhibitor resistance in hepatocellular carcinoma. Article Snippet: Tyrosine kinase inhibitor (TKI) resistance limits therapy for hepatocellular carcinoma (HCC).. Integrating RNAseq and public cohort data, we found consistent downregulation of the bile salt export pump (BSEP/ABCB11) in TKI-resistant HCC associated with poorer prognosis and reduced clinical response.. Functional in vitro and xenograft studies, using BSEP overexpression/knockdown and TKI-resistant cell lines plus targeted metabolomics, showed BSEP expression deficiency leads to intracellular accumulation of primary conjugated bile acids (BAs)—especially glycocholic acid (GCA)—which activates EGFR signaling and drives resistance; restoring BSEP enhances BA efflux and resensitizes cells and tumors to TKIs. Article Title: A bispecific nanobody-drug conjugate targeting TROP2 and c-Met for low-concentration, single-dose treatment of pancreatic cancer. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alexa Fluor® 700-anti human TROP2 antibody R&D Systems Cat#FAB650N-100UG; RRID: AB_3651666 PE-anti human c-Met antibody Abcam Cat#ab279587; Temporary RRID:AB_3740207 TACSTD2/TROP2 Monoclonal antibody Wuhan Sanying Cat#68141-1-Ig; CloneNo.5A6B1; RRID: AB_3085043 c-Met (Cytoplasmic) Polyclonal antibody Wuhan Sanying Cat#25869-1-AP; RRID: AB_2880276 Goat anti-human IgG Fc (HRP) Abcam Cat#ab97225; RRID: AB_10680850 HRP conjugated Goat Anti-Mouse IgG (H + L) Servicebio Cat#GB23301; RRID: AB_2904020 HRP conjugated Goat Anti-Rabbit IgG (H + L) Servicebio Cat#GB23303; RRID: AB_2811189 DyLight 650-Goat anti human IgG Fc Thermo Fisher RRID: AB_2556717 Rabbit anti-MMAE antibody(YA899) MedChemExpress (MCE) RRID: AB_3103046 F(ab’)2-Goat anti-Rabbit IgG (H + L) CrossAdsorbed Secondary Antibody, Alexa Fluor TM 647 Thermo Fisher RRID: AB_2535814 Goat anti-Human IgG (H + L) CrossAdsorbed Secondary Antibody, Alexa Fluor TM 488 Thermo Fisher RRID: AB_2534080 Cleaved Caspase-3 (Asp175) Antibody #9661Cleaved-Caspase3 Cell Signaling Technology (CST) Cat#9661S; RRID: AB_2341188 Cleaved Caspase-9 (Asp330) (D2D4) Rabbit mAb #7237 Cell Signaling Technology (CST) Cat#7237T; RRID: AB_10895832 Recombinant Anti-Caspase-7 antibody (Mouse mAb) Servicebio Cat#GB15540-100; Temporary RRID: AB_3740208 Anti-Cleaved-Caspase-3 Rabbit pAb Servicebio Cat#GB11532-100; RRID: AB_3096316 Anti-Cleaved PARP1 Rabbit pAb Servicebio Cat#GB111503-100; RRID: AB_3711714 Anti-Ki67 Rabbit pAb Servicebio Cat#GB111499-100; RRID: AB_2927572 Anti-PCNA Mouse mAb Servicebio Cat#GB12010-100; RRID: AB_3714765 Bacterial and virus strains DH5α Competent Cells Shenzhen Comedi Life Sciences Cat#KTSM101L ER2738 Electrocompetent Cells Lucigen Cat#60522-1 Biological samples Multi-Organ Cancer Tissue Microarray (TMA) Shanghai Outdo Biotech Cat#HOrgC180PG01-1 Pancreatic Cancer Tissue Microarray (TMA) Shanghai Outdo Biotech Cat#HPanA120PG02 Normal Human Multi-Organ Tissue Microarray (TMA) Shanghai Outdo Biotech Cat#HOrgN120PT02 Patient-derived xenografts (PDX) Nanchang Royo Biotech Patient ID: RYP-748; Patient ID: RYP-1301 Chemicals, peptides, and recombinant proteins Monomethyl auristatin E(MMAE) MedChemExpress (MCE) HY-15162; CAS No.474645-27-7 mc-vc-PABC-MMAE MedChemExpress (MCE) HY-15575; CAS No.646502-53-6 Cyanine5.5 NHS ester Luminprobe Cat#47020 DyLight TM 633 NHS Ester Thermo Fisher Cat#46414 Beetle Luciferin, Potassium Salt Promega Cat#E1601 (Continued on next page) Cell Reports Medicine 7, 102688, April 21, 2026 e1 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Phalloidin Labeling Probes Thermo Fisher Scientific Cat#A12379 PageRuler TM Prestained Protein Ladder Thermo Fisher Scientific Cat#26616 Sacituzumab govitecan MedChemExpress (MCE) HY-132254; CAS No.1491917-83-9 Telisotuzumab vedotin MedChemExpress (MCE) HY-141601; CAS No.1714088-51-3 Cathepsin B Protein, Human (His) MedChemExpress (MCE) Cat#HY-P70830 TROP2 Protein, Human, Recombinant (His Tag) Sinobiological Cat#10428-H08H c-MET Protein, Human, Recombinant (ECD, His Tag) Sinobiological Cat#10692-H08H Critical commercial assays Opal 7-color Manual IHC Kit BIOMART.CN Cat#NEL811001KT Chick Chorioallantoic Membrane Assay:Article Title: QD394 induces ferroptosis and suppresses the proliferation of colorectal cancer via the SP1/JNK pathway. Article Snippet: 1 Department of Gastroenterology, Yanbian University Hospital, Yanji 133002, China 2 Central Laboratory, Yanbian University Hospital, Yanji 133002, China Abstract Lipid peroxidation triggers ferroptosis, a type of regulated cell death that is iron-dependent.. Owing to its important role in tumor suppression, ferroptosis represents an extremely promising therapeutic target for cancer.. QD394, a quinazolinonebased compound, was recently identified as a novel redox regulator with demonstrated cytotoxic and proapoptotic effects in pancreatic and breast cancer models. Membrane:Article Title: QD394 induces ferroptosis and suppresses the proliferation of colorectal cancer via the SP1/JNK pathway. Article Snippet: 1 Department of Gastroenterology, Yanbian University Hospital, Yanji 133002, China 2 Central Laboratory, Yanbian University Hospital, Yanji 133002, China Abstract Lipid peroxidation triggers ferroptosis, a type of regulated cell death that is iron-dependent.. Owing to its important role in tumor suppression, ferroptosis represents an extremely promising therapeutic target for cancer.. QD394, a quinazolinonebased compound, was recently identified as a novel redox regulator with demonstrated cytotoxic and proapoptotic effects in pancreatic and breast cancer models. CCK-8 Assay:Article Title: QD394 induces ferroptosis and suppresses the proliferation of colorectal cancer via the SP1/JNK pathway. Article Snippet: 1 Department of Gastroenterology, Yanbian University Hospital, Yanji 133002, China 2 Central Laboratory, Yanbian University Hospital, Yanji 133002, China Abstract Lipid peroxidation triggers ferroptosis, a type of regulated cell death that is iron-dependent.. Owing to its important role in tumor suppression, ferroptosis represents an extremely promising therapeutic target for cancer.. QD394, a quinazolinonebased compound, was recently identified as a novel redox regulator with demonstrated cytotoxic and proapoptotic effects in pancreatic and breast cancer models. Article Title: A bispecific nanobody-drug conjugate targeting TROP2 and c-Met for low-concentration, single-dose treatment of pancreatic cancer. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alexa Fluor® 700-anti human TROP2 antibody R&D Systems Cat#FAB650N-100UG; RRID: AB_3651666 PE-anti human c-Met antibody Abcam Cat#ab279587; Temporary RRID:AB_3740207 TACSTD2/TROP2 Monoclonal antibody Wuhan Sanying Cat#68141-1-Ig; CloneNo.5A6B1; RRID: AB_3085043 c-Met (Cytoplasmic) Polyclonal antibody Wuhan Sanying Cat#25869-1-AP; RRID: AB_2880276 Goat anti-human IgG Fc (HRP) Abcam Cat#ab97225; RRID: AB_10680850 HRP conjugated Goat Anti-Mouse IgG (H + L) Servicebio Cat#GB23301; RRID: AB_2904020 HRP conjugated Goat Anti-Rabbit IgG (H + L) Servicebio Cat#GB23303; RRID: AB_2811189 DyLight 650-Goat anti human IgG Fc Thermo Fisher RRID: AB_2556717 Rabbit anti-MMAE antibody(YA899) MedChemExpress (MCE) RRID: AB_3103046 F(ab’)2-Goat anti-Rabbit IgG (H + L) CrossAdsorbed Secondary Antibody, Alexa Fluor TM 647 Thermo Fisher RRID: AB_2535814 Goat anti-Human IgG (H + L) CrossAdsorbed Secondary Antibody, Alexa Fluor TM 488 Thermo Fisher RRID: AB_2534080 Cleaved Caspase-3 (Asp175) Antibody #9661Cleaved-Caspase3 Cell Signaling Technology (CST) Cat#9661S; RRID: AB_2341188 Cleaved Caspase-9 (Asp330) (D2D4) Rabbit mAb #7237 Cell Signaling Technology (CST) Cat#7237T; RRID: AB_10895832 Recombinant Anti-Caspase-7 antibody (Mouse mAb) Servicebio Cat#GB15540-100; Temporary RRID: AB_3740208 Anti-Cleaved-Caspase-3 Rabbit pAb Servicebio Cat#GB11532-100; RRID: AB_3096316 Anti-Cleaved PARP1 Rabbit pAb Servicebio Cat#GB111503-100; RRID: AB_3711714 Anti-Ki67 Rabbit pAb Servicebio Cat#GB111499-100; RRID: AB_2927572 Anti-PCNA Mouse mAb Servicebio Cat#GB12010-100; RRID: AB_3714765 Bacterial and virus strains DH5α Competent Cells Shenzhen Comedi Life Sciences Cat#KTSM101L ER2738 Electrocompetent Cells Lucigen Cat#60522-1 Biological samples Multi-Organ Cancer Tissue Microarray (TMA) Shanghai Outdo Biotech Cat#HOrgC180PG01-1 Pancreatic Cancer Tissue Microarray (TMA) Shanghai Outdo Biotech Cat#HPanA120PG02 Normal Human Multi-Organ Tissue Microarray (TMA) Shanghai Outdo Biotech Cat#HOrgN120PT02 Patient-derived xenografts (PDX) Nanchang Royo Biotech Patient ID: RYP-748; Patient ID: RYP-1301 Chemicals, peptides, and recombinant proteins Monomethyl auristatin E(MMAE) MedChemExpress (MCE) HY-15162; CAS No.474645-27-7 mc-vc-PABC-MMAE MedChemExpress (MCE) HY-15575; CAS No.646502-53-6 Cyanine5.5 NHS ester Luminprobe Cat#47020 DyLight TM 633 NHS Ester Thermo Fisher Cat#46414 Beetle Luciferin, Potassium Salt Promega Cat#E1601 (Continued on next page) Cell Reports Medicine 7, 102688, April 21, 2026 e1 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Phalloidin Labeling Probes Thermo Fisher Scientific Cat#A12379 PageRuler TM Prestained Protein Ladder Thermo Fisher Scientific Cat#26616 Sacituzumab govitecan MedChemExpress (MCE) HY-132254; CAS No.1491917-83-9 Telisotuzumab vedotin MedChemExpress (MCE) HY-141601; CAS No.1714088-51-3 Cathepsin B Protein, Human (His) MedChemExpress (MCE) Cat#HY-P70830 TROP2 Protein, Human, Recombinant (His Tag) Sinobiological Cat#10428-H08H c-MET Protein, Human, Recombinant (ECD, His Tag) Sinobiological Cat#10692-H08H Critical commercial assays Opal 7-color Manual IHC Kit BIOMART.CN Cat#NEL811001KT Article Title: An antibody-PROTAC conjugate targets BRD4/c-Myc/PD-L1 to enhance immunotherapy efficacy in triple-negative breast cancer. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antifade mounting medium (with DAPI) Beyotime Cat#P0131 DMSO Beyotime Cat#ST038 Lipofectamine 3000 transfection reagent Thermo Fisher Scientific Cat#L3000015 Fixable Viability Dye eFluorTM 780 Thermo Fisher Scientific Cat#65-0865-14 Puromycin Beyotime Cat#ST551 DNase I Beyotime Cat#D7076 Collagenase Type IV Sigma Cat#C4-28 Percoll Sigma Cat#P4937 Cell Stimulation Cocktail (plus protein transport inhibitors) (500×) Thermo Fisher Scientific Cat#00-4975-93 Foxp3/Transcription Factor Staining Fixation/ Permeabilization Buffer Thermo Fisher Scientific Cat#00-5523-00 Real-time Polymerase Chain Reaction:Article Title: QD394 induces ferroptosis and suppresses the proliferation of colorectal cancer via the SP1/JNK pathway. Article Snippet: 1 Department of Gastroenterology, Yanbian University Hospital, Yanji 133002, China 2 Central Laboratory, Yanbian University Hospital, Yanji 133002, China Abstract Lipid peroxidation triggers ferroptosis, a type of regulated cell death that is iron-dependent.. Owing to its important role in tumor suppression, ferroptosis represents an extremely promising therapeutic target for cancer.. QD394, a quinazolinonebased compound, was recently identified as a novel redox regulator with demonstrated cytotoxic and proapoptotic effects in pancreatic and breast cancer models. Article Title: An antibody-PROTAC conjugate targets BRD4/c-Myc/PD-L1 to enhance immunotherapy efficacy in triple-negative breast cancer. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antifade mounting medium (with DAPI) Beyotime Cat#P0131 DMSO Beyotime Cat#ST038 Lipofectamine 3000 transfection reagent Thermo Fisher Scientific Cat#L3000015 Fixable Viability Dye eFluorTM 780 Thermo Fisher Scientific Cat#65-0865-14 Puromycin Beyotime Cat#ST551 DNase I Beyotime Cat#D7076 Collagenase Type IV Sigma Cat#C4-28 Percoll Sigma Cat#P4937 Cell Stimulation Cocktail (plus protein transport inhibitors) (500×) Thermo Fisher Scientific Cat#00-4975-93 Foxp3/Transcription Factor Staining Fixation/ Permeabilization Buffer Thermo Fisher Scientific Cat#00-5523-00 Western Blot:Article Title: QD394 induces ferroptosis and suppresses the proliferation of colorectal cancer via the SP1/JNK pathway. Article Snippet: 1 Department of Gastroenterology, Yanbian University Hospital, Yanji 133002, China 2 Central Laboratory, Yanbian University Hospital, Yanji 133002, China Abstract Lipid peroxidation triggers ferroptosis, a type of regulated cell death that is iron-dependent.. Owing to its important role in tumor suppression, ferroptosis represents an extremely promising therapeutic target for cancer.. QD394, a quinazolinonebased compound, was recently identified as a novel redox regulator with demonstrated cytotoxic and proapoptotic effects in pancreatic and breast cancer models. Labeling:Article Title: A bispecific nanobody-drug conjugate targeting TROP2 and c-Met for low-concentration, single-dose treatment of pancreatic cancer. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alexa Fluor® 700-anti human TROP2 antibody R&D Systems Cat#FAB650N-100UG; RRID: AB_3651666 PE-anti human c-Met antibody Abcam Cat#ab279587; Temporary RRID:AB_3740207 TACSTD2/TROP2 Monoclonal antibody Wuhan Sanying Cat#68141-1-Ig; CloneNo.5A6B1; RRID: AB_3085043 c-Met (Cytoplasmic) Polyclonal antibody Wuhan Sanying Cat#25869-1-AP; RRID: AB_2880276 Goat anti-human IgG Fc (HRP) Abcam Cat#ab97225; RRID: AB_10680850 HRP conjugated Goat Anti-Mouse IgG (H + L) Servicebio Cat#GB23301; RRID: AB_2904020 HRP conjugated Goat Anti-Rabbit IgG (H + L) Servicebio Cat#GB23303; RRID: AB_2811189 DyLight 650-Goat anti human IgG Fc Thermo Fisher RRID: AB_2556717 Rabbit anti-MMAE antibody(YA899) MedChemExpress (MCE) RRID: AB_3103046 F(ab’)2-Goat anti-Rabbit IgG (H + L) CrossAdsorbed Secondary Antibody, Alexa Fluor TM 647 Thermo Fisher RRID: AB_2535814 Goat anti-Human IgG (H + L) CrossAdsorbed Secondary Antibody, Alexa Fluor TM 488 Thermo Fisher RRID: AB_2534080 Cleaved Caspase-3 (Asp175) Antibody #9661Cleaved-Caspase3 Cell Signaling Technology (CST) Cat#9661S; RRID: AB_2341188 Cleaved Caspase-9 (Asp330) (D2D4) Rabbit mAb #7237 Cell Signaling Technology (CST) Cat#7237T; RRID: AB_10895832 Recombinant Anti-Caspase-7 antibody (Mouse mAb) Servicebio Cat#GB15540-100; Temporary RRID: AB_3740208 Anti-Cleaved-Caspase-3 Rabbit pAb Servicebio Cat#GB11532-100; RRID: AB_3096316 Anti-Cleaved PARP1 Rabbit pAb Servicebio Cat#GB111503-100; RRID: AB_3711714 Anti-Ki67 Rabbit pAb Servicebio Cat#GB111499-100; RRID: AB_2927572 Anti-PCNA Mouse mAb Servicebio Cat#GB12010-100; RRID: AB_3714765 Bacterial and virus strains DH5α Competent Cells Shenzhen Comedi Life Sciences Cat#KTSM101L ER2738 Electrocompetent Cells Lucigen Cat#60522-1 Biological samples Multi-Organ Cancer Tissue Microarray (TMA) Shanghai Outdo Biotech Cat#HOrgC180PG01-1 Pancreatic Cancer Tissue Microarray (TMA) Shanghai Outdo Biotech Cat#HPanA120PG02 Normal Human Multi-Organ Tissue Microarray (TMA) Shanghai Outdo Biotech Cat#HOrgN120PT02 Patient-derived xenografts (PDX) Nanchang Royo Biotech Patient ID: RYP-748; Patient ID: RYP-1301 Chemicals, peptides, and recombinant proteins Monomethyl auristatin E(MMAE) MedChemExpress (MCE) HY-15162; CAS No.474645-27-7 mc-vc-PABC-MMAE MedChemExpress (MCE) HY-15575; CAS No.646502-53-6 Cyanine5.5 NHS ester Luminprobe Cat#47020 DyLight TM 633 NHS Ester Thermo Fisher Cat#46414 Beetle Luciferin, Potassium Salt Promega Cat#E1601 (Continued on next page) Cell Reports Medicine 7, 102688, April 21, 2026 e1 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Phalloidin Labeling Probes Thermo Fisher Scientific Cat#A12379 PageRuler TM Prestained Protein Ladder Thermo Fisher Scientific Cat#26616 Sacituzumab govitecan MedChemExpress (MCE) HY-132254; CAS No.1491917-83-9 Telisotuzumab vedotin MedChemExpress (MCE) HY-141601; CAS No.1714088-51-3 Cathepsin B Protein, Human (His) MedChemExpress (MCE) Cat#HY-P70830 TROP2 Protein, Human, Recombinant (His Tag) Sinobiological Cat#10428-H08H c-MET Protein, Human, Recombinant (ECD, His Tag) Sinobiological Cat#10692-H08H Critical commercial assays Opal 7-color Manual IHC Kit BIOMART.CN Cat#NEL811001KT Recombinant:Article Title: A bispecific nanobody-drug conjugate targeting TROP2 and c-Met for low-concentration, single-dose treatment of pancreatic cancer. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alexa Fluor® 700-anti human TROP2 antibody R&D Systems Cat#FAB650N-100UG; RRID: AB_3651666 PE-anti human c-Met antibody Abcam Cat#ab279587; Temporary RRID:AB_3740207 TACSTD2/TROP2 Monoclonal antibody Wuhan Sanying Cat#68141-1-Ig; CloneNo.5A6B1; RRID: AB_3085043 c-Met (Cytoplasmic) Polyclonal antibody Wuhan Sanying Cat#25869-1-AP; RRID: AB_2880276 Goat anti-human IgG Fc (HRP) Abcam Cat#ab97225; RRID: AB_10680850 HRP conjugated Goat Anti-Mouse IgG (H + L) Servicebio Cat#GB23301; RRID: AB_2904020 HRP conjugated Goat Anti-Rabbit IgG (H + L) Servicebio Cat#GB23303; RRID: AB_2811189 DyLight 650-Goat anti human IgG Fc Thermo Fisher RRID: AB_2556717 Rabbit anti-MMAE antibody(YA899) MedChemExpress (MCE) RRID: AB_3103046 F(ab’)2-Goat anti-Rabbit IgG (H + L) CrossAdsorbed Secondary Antibody, Alexa Fluor TM 647 Thermo Fisher RRID: AB_2535814 Goat anti-Human IgG (H + L) CrossAdsorbed Secondary Antibody, Alexa Fluor TM 488 Thermo Fisher RRID: AB_2534080 Cleaved Caspase-3 (Asp175) Antibody #9661Cleaved-Caspase3 Cell Signaling Technology (CST) Cat#9661S; RRID: AB_2341188 Cleaved Caspase-9 (Asp330) (D2D4) Rabbit mAb #7237 Cell Signaling Technology (CST) Cat#7237T; RRID: AB_10895832 Recombinant Anti-Caspase-7 antibody (Mouse mAb) Servicebio Cat#GB15540-100; Temporary RRID: AB_3740208 Anti-Cleaved-Caspase-3 Rabbit pAb Servicebio Cat#GB11532-100; RRID: AB_3096316 Anti-Cleaved PARP1 Rabbit pAb Servicebio Cat#GB111503-100; RRID: AB_3711714 Anti-Ki67 Rabbit pAb Servicebio Cat#GB111499-100; RRID: AB_2927572 Anti-PCNA Mouse mAb Servicebio Cat#GB12010-100; RRID: AB_3714765 Bacterial and virus strains DH5α Competent Cells Shenzhen Comedi Life Sciences Cat#KTSM101L ER2738 Electrocompetent Cells Lucigen Cat#60522-1 Biological samples Multi-Organ Cancer Tissue Microarray (TMA) Shanghai Outdo Biotech Cat#HOrgC180PG01-1 Pancreatic Cancer Tissue Microarray (TMA) Shanghai Outdo Biotech Cat#HPanA120PG02 Normal Human Multi-Organ Tissue Microarray (TMA) Shanghai Outdo Biotech Cat#HOrgN120PT02 Patient-derived xenografts (PDX) Nanchang Royo Biotech Patient ID: RYP-748; Patient ID: RYP-1301 Chemicals, peptides, and recombinant proteins Monomethyl auristatin E(MMAE) MedChemExpress (MCE) HY-15162; CAS No.474645-27-7 mc-vc-PABC-MMAE MedChemExpress (MCE) HY-15575; CAS No.646502-53-6 Cyanine5.5 NHS ester Luminprobe Cat#47020 DyLight TM 633 NHS Ester Thermo Fisher Cat#46414 Beetle Luciferin, Potassium Salt Promega Cat#E1601 (Continued on next page) Cell Reports Medicine 7, 102688, April 21, 2026 e1 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Phalloidin Labeling Probes Thermo Fisher Scientific Cat#A12379 PageRuler TM Prestained Protein Ladder Thermo Fisher Scientific Cat#26616 Sacituzumab govitecan MedChemExpress (MCE) HY-132254; CAS No.1491917-83-9 Telisotuzumab vedotin MedChemExpress (MCE) HY-141601; CAS No.1714088-51-3 Cathepsin B Protein, Human (His) MedChemExpress (MCE) Cat#HY-P70830 TROP2 Protein, Human, Recombinant (His Tag) Sinobiological Cat#10428-H08H c-MET Protein, Human, Recombinant (ECD, His Tag) Sinobiological Cat#10692-H08H Critical commercial assays Opal 7-color Manual IHC Kit BIOMART.CN Cat#NEL811001KT Article Title: An antibody-PROTAC conjugate targets BRD4/c-Myc/PD-L1 to enhance immunotherapy efficacy in triple-negative breast cancer. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antifade mounting medium (with DAPI) Beyotime Cat#P0131 DMSO Beyotime Cat#ST038 Lipofectamine 3000 transfection reagent Thermo Fisher Scientific Cat#L3000015 Fixable Viability Dye eFluorTM 780 Thermo Fisher Scientific Cat#65-0865-14 Puromycin Beyotime Cat#ST551 DNase I Beyotime Cat#D7076 Collagenase Type IV Sigma Cat#C4-28 Percoll Sigma Cat#P4937 Cell Stimulation Cocktail (plus protein transport inhibitors) (500×) Thermo Fisher Scientific Cat#00-4975-93 Foxp3/Transcription Factor Staining Fixation/ Permeabilization Buffer Thermo Fisher Scientific Cat#00-5523-00 Expressing:Article Title: A bispecific nanobody-drug conjugate targeting TROP2 and c-Met for low-concentration, single-dose treatment of pancreatic cancer. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alexa Fluor® 700-anti human TROP2 antibody R&D Systems Cat#FAB650N-100UG; RRID: AB_3651666 PE-anti human c-Met antibody Abcam Cat#ab279587; Temporary RRID:AB_3740207 TACSTD2/TROP2 Monoclonal antibody Wuhan Sanying Cat#68141-1-Ig; CloneNo.5A6B1; RRID: AB_3085043 c-Met (Cytoplasmic) Polyclonal antibody Wuhan Sanying Cat#25869-1-AP; RRID: AB_2880276 Goat anti-human IgG Fc (HRP) Abcam Cat#ab97225; RRID: AB_10680850 HRP conjugated Goat Anti-Mouse IgG (H + L) Servicebio Cat#GB23301; RRID: AB_2904020 HRP conjugated Goat Anti-Rabbit IgG (H + L) Servicebio Cat#GB23303; RRID: AB_2811189 DyLight 650-Goat anti human IgG Fc Thermo Fisher RRID: AB_2556717 Rabbit anti-MMAE antibody(YA899) MedChemExpress (MCE) RRID: AB_3103046 F(ab’)2-Goat anti-Rabbit IgG (H + L) CrossAdsorbed Secondary Antibody, Alexa Fluor TM 647 Thermo Fisher RRID: AB_2535814 Goat anti-Human IgG (H + L) CrossAdsorbed Secondary Antibody, Alexa Fluor TM 488 Thermo Fisher RRID: AB_2534080 Cleaved Caspase-3 (Asp175) Antibody #9661Cleaved-Caspase3 Cell Signaling Technology (CST) Cat#9661S; RRID: AB_2341188 Cleaved Caspase-9 (Asp330) (D2D4) Rabbit mAb #7237 Cell Signaling Technology (CST) Cat#7237T; RRID: AB_10895832 Recombinant Anti-Caspase-7 antibody (Mouse mAb) Servicebio Cat#GB15540-100; Temporary RRID: AB_3740208 Anti-Cleaved-Caspase-3 Rabbit pAb Servicebio Cat#GB11532-100; RRID: AB_3096316 Anti-Cleaved PARP1 Rabbit pAb Servicebio Cat#GB111503-100; RRID: AB_3711714 Anti-Ki67 Rabbit pAb Servicebio Cat#GB111499-100; RRID: AB_2927572 Anti-PCNA Mouse mAb Servicebio Cat#GB12010-100; RRID: AB_3714765 Bacterial and virus strains DH5α Competent Cells Shenzhen Comedi Life Sciences Cat#KTSM101L ER2738 Electrocompetent Cells Lucigen Cat#60522-1 Biological samples Multi-Organ Cancer Tissue Microarray (TMA) Shanghai Outdo Biotech Cat#HOrgC180PG01-1 Pancreatic Cancer Tissue Microarray (TMA) Shanghai Outdo Biotech Cat#HPanA120PG02 Normal Human Multi-Organ Tissue Microarray (TMA) Shanghai Outdo Biotech Cat#HOrgN120PT02 Patient-derived xenografts (PDX) Nanchang Royo Biotech Patient ID: RYP-748; Patient ID: RYP-1301 Chemicals, peptides, and recombinant proteins Monomethyl auristatin E(MMAE) MedChemExpress (MCE) HY-15162; CAS No.474645-27-7 mc-vc-PABC-MMAE MedChemExpress (MCE) HY-15575; CAS No.646502-53-6 Cyanine5.5 NHS ester Luminprobe Cat#47020 DyLight TM 633 NHS Ester Thermo Fisher Cat#46414 Beetle Luciferin, Potassium Salt Promega Cat#E1601 (Continued on next page) Cell Reports Medicine 7, 102688, April 21, 2026 e1 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Phalloidin Labeling Probes Thermo Fisher Scientific Cat#A12379 PageRuler TM Prestained Protein Ladder Thermo Fisher Scientific Cat#26616 Sacituzumab govitecan MedChemExpress (MCE) HY-132254; CAS No.1491917-83-9 Telisotuzumab vedotin MedChemExpress (MCE) HY-141601; CAS No.1714088-51-3 Cathepsin B Protein, Human (His) MedChemExpress (MCE) Cat#HY-P70830 TROP2 Protein, Human, Recombinant (His Tag) Sinobiological Cat#10428-H08H c-MET Protein, Human, Recombinant (ECD, His Tag) Sinobiological Cat#10692-H08H Critical commercial assays Opal 7-color Manual IHC Kit BIOMART.CN Cat#NEL811001KT Transfection:Article Title: An antibody-PROTAC conjugate targets BRD4/c-Myc/PD-L1 to enhance immunotherapy efficacy in triple-negative breast cancer. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antifade mounting medium (with DAPI) Beyotime Cat#P0131 DMSO Beyotime Cat#ST038 Lipofectamine 3000 transfection reagent Thermo Fisher Scientific Cat#L3000015 Fixable Viability Dye eFluorTM 780 Thermo Fisher Scientific Cat#65-0865-14 Puromycin Beyotime Cat#ST551 DNase I Beyotime Cat#D7076 Collagenase Type IV Sigma Cat#C4-28 Percoll Sigma Cat#P4937 Cell Stimulation Cocktail (plus protein transport inhibitors) (500×) Thermo Fisher Scientific Cat#00-4975-93 Foxp3/Transcription Factor Staining Fixation/ Permeabilization Buffer Thermo Fisher Scientific Cat#00-5523-00 Cell Stimulation:Article Title: An antibody-PROTAC conjugate targets BRD4/c-Myc/PD-L1 to enhance immunotherapy efficacy in triple-negative breast cancer. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antifade mounting medium (with DAPI) Beyotime Cat#P0131 DMSO Beyotime Cat#ST038 Lipofectamine 3000 transfection reagent Thermo Fisher Scientific Cat#L3000015 Fixable Viability Dye eFluorTM 780 Thermo Fisher Scientific Cat#65-0865-14 Puromycin Beyotime Cat#ST551 DNase I Beyotime Cat#D7076 Collagenase Type IV Sigma Cat#C4-28 Percoll Sigma Cat#P4937 Cell Stimulation Cocktail (plus protein transport inhibitors) (500×) Thermo Fisher Scientific Cat#00-4975-93 Foxp3/Transcription Factor Staining Fixation/ Permeabilization Buffer Thermo Fisher Scientific Cat#00-5523-00 Sequencing:Article Title: An antibody-PROTAC conjugate targets BRD4/c-Myc/PD-L1 to enhance immunotherapy efficacy in triple-negative breast cancer. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antifade mounting medium (with DAPI) Beyotime Cat#P0131 DMSO Beyotime Cat#ST038 Lipofectamine 3000 transfection reagent Thermo Fisher Scientific Cat#L3000015 Fixable Viability Dye eFluorTM 780 Thermo Fisher Scientific Cat#65-0865-14 Puromycin Beyotime Cat#ST551 DNase I Beyotime Cat#D7076 Collagenase Type IV Sigma Cat#C4-28 Percoll Sigma Cat#P4937 Cell Stimulation Cocktail (plus protein transport inhibitors) (500×) Thermo Fisher Scientific Cat#00-4975-93 Foxp3/Transcription Factor Staining Fixation/ Permeabilization Buffer Thermo Fisher Scientific Cat#00-5523-00 Quantitative RT-PCR:Article Title: An antibody-PROTAC conjugate targets BRD4/c-Myc/PD-L1 to enhance immunotherapy efficacy in triple-negative breast cancer. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antifade mounting medium (with DAPI) Beyotime Cat#P0131 DMSO Beyotime Cat#ST038 Lipofectamine 3000 transfection reagent Thermo Fisher Scientific Cat#L3000015 Fixable Viability Dye eFluorTM 780 Thermo Fisher Scientific Cat#65-0865-14 Puromycin Beyotime Cat#ST551 DNase I Beyotime Cat#D7076 Collagenase Type IV Sigma Cat#C4-28 Percoll Sigma Cat#P4937 Cell Stimulation Cocktail (plus protein transport inhibitors) (500×) Thermo Fisher Scientific Cat#00-4975-93 Foxp3/Transcription Factor Staining Fixation/ Permeabilization Buffer Thermo Fisher Scientific Cat#00-5523-00 Software:Article Title: An antibody-PROTAC conjugate targets BRD4/c-Myc/PD-L1 to enhance immunotherapy efficacy in triple-negative breast cancer. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antifade mounting medium (with DAPI) Beyotime Cat#P0131 DMSO Beyotime Cat#ST038 Lipofectamine 3000 transfection reagent Thermo Fisher Scientific Cat#L3000015 Fixable Viability Dye eFluorTM 780 Thermo Fisher Scientific Cat#65-0865-14 Puromycin Beyotime Cat#ST551 DNase I Beyotime Cat#D7076 Collagenase Type IV Sigma Cat#C4-28 Percoll Sigma Cat#P4937 Cell Stimulation Cocktail (plus protein transport inhibitors) (500×) Thermo Fisher Scientific Cat#00-4975-93 Foxp3/Transcription Factor Staining Fixation/ Permeabilization Buffer Thermo Fisher Scientific Cat#00-5523-00 |
